PMC

The strains and their corresponding NCBI accession numbers: Bacillus hemicellulosilyticus DSM 16731^T AB043846; Bacillus halodurans XJU-2 AY960851; Bacillus akibai ATCC 43226^T AB043858; Bacillus macyae DSM 16346^T AY032601; Bacillus alkalidiazotrophicus UNIQEM U377^T EU143680; Bacillus alkalinitrilicus UNIQEM U240^T EF422411; Bacillus algicola LS7 FJ937877; Bacillus mannanilyticus DSM 16130^T AB043864; Bacillus pseudofirmus SVB1 EU533950; Bacillus cellulosilyticus DSM 2522^T AB043852; Bacillus vedderi DSM 9768^T Z48306; Bacillus polygoni JCM 14604^T AB292819; Bacillus clarkia DSM 8720^T X76444; Bacillus agaradhaerens GSP78 AY553093; Bacillus aurantiacus B1-1 AJ606036; Bacillus amyloliquefaciens CMB01 AF489591; Bacillus subtilis AY887082; Bacillus licheniformis 11 FJ435222; and Bacillus alveayuensis KCTC 10634^T AY605232.

Reference mass spectra from Bacillus tuaregi strain Marseille-P2489^T, Bacillus massilinigeriensis strain Marseille-P2366^T, Bacillus phocaeensis strain SIT16^T, Bacillus massiliglaciei strain Marseille-P2600^T and Bacillus mediterraneensis strain Marseille-P2384^T. Spectra from 12 individual colonies were compared and each reference spectrum generated (A). Gel view comparing Bacillus tuaregi strain Marseille-P2489^T, Bacillus massilinigeriensis strain Marseille-P2366^T, Bacillus phocaeensis strain SIT16^T, Bacillus massiliglaciei strain Marseille-P2600^T and Bacillus mediterraneensis strain Marseille-P2384^T to other species within genus Bacillus. Gel view displays raw spectra of loaded spectrum files arranged in pseudo-gel-like look. x-axis records m/z value, left y-axis running spectrum number originating from subsequent spectra loading. Peak intensity expressed by greyscale scheme code. Color bar and right y-axis indicate relation between color peaks, with peak intensity in arbitrary units. Displayed species are indicated at left (B).

Proteins are listed using NCBI locus tags. Bootstrap values are shown for selected branches and correspond to confidence levels. A) Black: B. subtilis 168 FosB (outgroup), green: Bacillus subtilis 168 proteins, orange: Bacillus subtilis subsp. natto BEST195 proteins, magenta: Bacillus subtilis subsp. spizizenii TU-B-10 proteins, cyan: Bacillus subtilis subsp. spizizenii str. W23 proteins, red: Bacillus subtilis subsp. subtilis str. RO-NN-1 proteins See Table A in for additional information. B) Black: B. subtilis 168 FosB (outgroup), green: Bacillus subtilis 168 proteins, orange: Bacillus megaterium QM B1551 proteins, purple: Bacillus amyloliquefaciens subsp. plantarum str. FZB42 proteins, cyan: Bacillus halodurans C-125 proteins, red: Bacillus anthracis str. Ames, blue: Bacillus thuringiensis str. Al Hakam. See Table B in for additional information.

Selective identification of Bacillus thuringiensis colonies on Bacillus agar. Bacillus thuringiensis colonies were grown as blue/green in color (A), Bacillus cereus appeared as white color colonies with a light blue center (B), and Bacillus megaterium colonies appeared yellow (C).